The purpose of this study was to investigate the differences of driving performance and physiological responses between young male and female drivers for unexpected situation using a graphic vehicle simulator. The participants included 20 college graduates; 23 males aged 24.3 ± 1.4 with 2.3 ± 1.5 years of driving experiences and 23 females aged 23.2 ± 2.1with 22 ± 1.7 years of driving experience. The participants were instructed to drive the vehicle simulator which was programed unexpected situation for two minutes. The physiological measurements used were autonomic responses of electrocardiogram (ECG) and skin conductance response (SCR), and the driving performance measurements used were the reaction time of break and the rate of collision for unexpected situation. Results showed that there were no significant differences between male and female drivers in the reaction time of break and the rate of collision for unexpected situation. Averaged R-R interval decreased and LF IHF and SCL amplitude increased for unexpected situation. There were no significant differences between male and female in the averaged R-R interval and LF/HF for unexpected situation. On the other hand, SCL amplitude of female was higher than male. Rising time to maximum SCL amplitude of female was longer than male.