In this study, reactions of central nervous systems working against different conditions of forced frequency and acceleration were measured and analyzed. The experiment are conducted with health men. The steady vibration conditions of forced frequency (0.315m/s2-1.0Hz, 0.315m/s2-10Hz and 10Hz-1.0m/s2) are used and the waves of EEG (Electroencephalogram) are measured. As a result, this paper shows that the α-wave of frontal lobe transfers from low to high frequency band under the vibration environment. Additionally, the average frequency of α-wave is higher under the vibration than under non-vibration environment. In the case of forced frequency of 1.0Hz-0.315m/s2, the feeling with the vibration are nearly same compared with the non-vibration condition. But in the case of 10Hz-1.0m/s2, uncomfortable feeling increased compared with the non-vibration condition. This study also shows the relationship between fluctuation slop and feeling. From this study, it is found that the effect of vibration on human depends on acceleration characteristics. Highly accelerating vibration is more harmful to human.